|本期目录/Table of Contents|

[1]蔡琳,马凌,张卫泽,等.5-氮杂胞苷通过PI3K/Akt/mTOR通路介导人脂肪间充质干细胞向心肌细胞定向分化的研究[J].国际心血管病杂志,2015,05:348-351.
　CAI Lin,MA Ling,ZHANG Weize,et al.5-Azacytidine induces differentiation of adult adipose-derived mesenchymal stem cells to cardiomyocyte-like cells through PI3K/Akt/mTOR signaling pathway[J].International Journal of Cardiovascular Disease,2015,05:348-351.
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5-氮杂胞苷通过PI3K/Akt/mTOR通路介导人脂肪间充质干细胞向心肌细胞定向分化的研究(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2015年05期
页码:
348-351
栏目:
出版日期:
2015-09-25

文章信息/Info

Title:
5-Azacytidine induces differentiation of adult adipose-derived mesenchymal stem cells to cardiomyocyte-like cells through PI3K/Akt/mTOR signaling pathway
作者:
蔡琳; 马凌; 张卫泽; 王菲; 张汉平
730050 解放军兰州军区兰州总医院
Author(s):
CAI Lin;  MA Ling;  ZHANG Weize;  WANG Fei;  ZHANG Hanping.
Lanzhou General Hospital of Lanzhou Military Area Command, PLA, Gansu 730050, China
关键词:
PI3K/Akt/mTOR信号通路; 脂肪间充质干细胞; 5-氮杂胞苷; 细胞分化
Keywords:
PI3K/Akt/mTOR;  adipose-derived mesenchymal stem cells;  5-Azacytidine;  Cell differentiation
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2015.05.016
文献标识码:
-
摘要:
目的:研究磷脂酰肌醇3激酶(PI3K)及其下游分子丝氨酸/苏氨酸激酶(Akt)和雷帕霉素靶蛋白(mTOR)所组成的信号通路在5-氮杂胞苷(5-aza)诱导人脂肪间充质干细胞(ADMSCs)向心肌细胞定向分化中的作用,探讨其信号转导机制。方法:利用胶原酶法分离、培养ADMSCs,并用10μmol/L的5-aza诱导其向心肌细胞定向分化,采用Westernblot的方法分析5-aza诱导前后Akt通路相关蛋白的表达情况。结果:5-aza诱导前ADMSCs内Akt通路相关蛋白的表达水平较低,诱导后增强。PI3K
Abstract:
Objective: To investigate the regulating effects of PI3K/Akt signaling pathway on the differentiation of the adult adipose-derived mesenchymal stem cells(ADMSCs)induced with 5-Azacytidine(5-aza)toward cardiomyocytes and to determine the possible mechanism

参考文献/References

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备注/Memo

备注/Memo:
基金项目:甘肃省自然科学基金(1208RJZA213) 作者单位:730050 解放军兰州军区兰州总医院 通信作者:马 凌,Email: maling4079@sina.com
更新日期/Last Update: 2015-10-20