|本期目录/Table of Contents|

[1]陆佳,蔡利栋,吴晓宇,等.肌成纤维细胞来源外泌体介导主动脉缩窄后 心脏重构的作用[J].国际心血管病杂志,2022,03:169-175.
　LU JiaCAI Lidong,WU Xiaoyu,DING Yu,et al.Effects of myofibroblasts-derived exosomes on cardiac remodeling post transverse aortic constriction[J].International Journal of Cardiovascular Disease,2022,03:169-175.
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肌成纤维细胞来源外泌体介导主动脉缩窄后 心脏重构的作用(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2022年03期
页码:
169-175
栏目:
基础研究
出版日期:
2022-06-20

文章信息/Info

Title:
Effects of myofibroblasts-derived exosomes on cardiac remodeling post transverse aortic constriction
作者:
陆佳; 蔡利栋; 吴晓宇; 丁羽; 巩超
314100 浙江省嘉兴市嘉善县第一人民医院心内科
Author(s):
LU Jia; CAI Lidong;  WU Xiaoyu;  DING Yu;  GONG Chao
Department of cardiology, The first people’ s hospital of Jia shan, Zhejiang 314100
关键词:
主动脉缩窄; 成纤维细胞; 心脏重构; 外泌体
Keywords:
Transverse aortic constriction;  Cardiac fibroblasts;  Cardiac remodeling;  Exosomes
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2022.03.010
文献标识码:
-
摘要:
目的：观察肌成纤维细胞来源外泌体对主动脉缩窄后心脏重构的影响。方法： 利用促纤维化因子转化生长因子-β（TGF-β）诱导心脏成纤维细胞活化，将原代心肌细胞 分为正常组、PBS 组、外泌体组，通过超高速离心分离提取成纤维细胞上清液中的外泌体， 并将其与外泌体组心肌细胞共同孵育48 h。实时定量聚合酶链反应及免疫荧光染色法检测 心肌细胞肥厚指标（心房利钠肽、脑钠肽和β 肌球蛋白重链）以及心肌细胞表面积。选取 C57 雄性小鼠32 只，随机分为对照组、假手术组、主动脉缩窄- 对照组、主动脉缩窄- 外泌 体组，每
Abstract:
Objective: To explore the role of exosomes derived from myofibroblasts in cardiac remodeling post transverse aortic constriction (TAC). Methods: Exosomes isolated from supernatant of cardiac fibroblasts (CFs) stimulated by TGF-β were incubated with cardio

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（82000332）; 上海交通大学医工交叉研究项目（YG2019QNA56）
通信作者：蔡利栋， E-mail: lidong.cai@shgh.cnn
更新日期/Last Update: 2022-06-20