|本期目录/Table of Contents|

[1]查晴,肖凡,张倩茹,等.主动脉瓣膜钙化中胰高血糖素样肽-1对Notch1-Sox9信号通路的调控作用[J].国际心血管病杂志,2021,06:370-375,379.
 ZHA Qing,XIAO Fan,ZHANG QianRu,et al.The regulatory effects of glucagon-like peptide-1 on Notch1-Sox9 signaling pathway in aortic valve calcification[J].International Journal of Cardiovascular Disease,2021,06:370-375,379.
点击复制

主动脉瓣膜钙化中胰高血糖素样肽-1对Notch1-Sox9信号通路的调控作用(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2021年06期
页码:
370-375,379
栏目:
基础研究
出版日期:
2021-12-01

文章信息/Info

Title:
The regulatory effects of glucagon-like peptide-1 on Notch1-Sox9 signaling pathway in aortic valve calcification
作者:
查晴肖凡张倩茹叶佳雯张煜杨玲杨克刘艳
200001 上海交通大学医学院附属第九人民医院心血管内科(查晴,肖凡,张倩茹,叶佳雯,张煜,杨玲,刘艳); 200025 上海交通大学医学院附属瑞金医院心血管内科(杨克)
Author(s):
ZHA Qing1 XIAO Fan1 ZHANG QianRu1 YE Jiawen1 ZHANG Yu1 YANG Ling1 YANG Ke2 LIU Yan1.
1.Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011; 2.Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
关键词:
胰高血糖素样肽-1受体 瓣膜间质细胞 钙化 Sox9 Notch胞内肽段
Keywords:
GLP-1 receptor Valve interstitial cell Calcification Sox9 Notch intracellular domain
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2021.06.013
文献标识码:
-
摘要:
目的:探讨胰高血糖素样肽-1(GLP-1)及GLP-1受体(GLP-1R)下游信号通路对主动脉瓣膜间质细胞钙化的调控作用。方法:收集16例因主动脉瓣病变行置换术患者(瓣膜钙化组)和20例因其他原因行心脏移植但瓣膜正常患者(对照组)的主动脉瓣。苏木精-伊红染色(HE染色)和茜素红染色法检测瓣膜结构变化及钙化情况,免疫组织化学染色检测GLP-1R表达情况。培养原代小鼠瓣膜间质细胞,分为正常培养组、钙化培养组和钙化培养+GLP-1组,茜素红染色检测钙化情况,实时荧光定量聚合酶链反应(qRT-PCR)检测S
Abstract:
Objective:To investigate the regulatory effects of glucagon-like peptide-1(GLP-1)and GLP-1 receptor(GLP-1R)on the calcification of aortic valve interstitial cells.Methods:The aortic valve tissues of 16 patients with aortic valve disease who underwent repl

参考文献/References

[1] de Oliveira Sá MPB, Cavalcanti LRP, Perazzo áM, et al. Calcific aortic valve stenosis and atherosclerotic calcification[J]. Curr Atheroscler Rep, 2020, 22(2):2.
[2] Kostyunin AE, Yuzhalin AE, Ovcharenko EA, et al. Development of calcific aortic valve disease: Do we know enough for new clinical trials?[J]. J Mol Cell Cardiol, 2019, 132:189-209.
[3] Carpenter TO, Shaw NJ, Portale AA, et al. Rickets[J]. Nat Rev Dis Primers, 2017, 3:17101.
[4] Johnson RC, Leopold JA, Loscalzo J. Vascular calcification: pathobiological mechanisms and clinical implications[J]. Circ Res, 2006, 99(10):1044-1059.
[5] Lindman BR, Clavel MA, Mathieu P, et al. Calcific aortic stenosis[J]. Nat Rev Dis Primers, 2016, 2:16006.
[6] Chaudhari S, Yazdizadeh Shotorbani P, Tao Y, et al. Inhibition of interleukin-6 on matrix protein production by glomerular mesangial cells and the pathway involved[J]. Am J Physiol Renal Physiol, 2020, 318:F1478-F1488.
[7] Laguna-Fernandez A, Carracedo M, Jeanson G, et al. Iron alters valvular interstitial cell function and is associated with calcification in aortic stenosis[J]. Eur Heart J, 2016, 37(47):3532-3535.
[8] Schlotter F, Halu A, Goto S, et al. Spatiotemporal multi-omics mapping generates a molecular atlas of the aortic valve and reveals networks driving disease[J]. Circulation, 2018, 138(4):377-393.
[9] Simard L, Côté N, Dagenais F, et al. Sex-related discordance between aortic valve calcification and hemodynamic severity of aortic stenosis: Is valvular fibrosis the explanation?[J]. Circ Res, 2017, 120(4):681-691.
[10] Choi JH, Kim SJ, Kwon SK, et al. Renal tubular glucagon-like peptide-1 receptor expression is increased in early sepsis but reduced in chronic kidney disease and sepsis-induced kidney injury[J]. Int J Mol Sci, 2019, 20(23):6024.
[11] Kanda R, Hiraike H, Wada-Hiraike O, et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer [J]. BMC Cancer, 2018, 18(1):657.
[12] Malbert CH, Chauvin A, Horowitz M, et al. Glucose sensing mediated by portal glucagon-like peptide 1 receptor is markedly impaired in insulin-resistant obese animals[J]. Diabetes, 2021, 70(1):99-110.
[13] Naruse G, Kanamori H, Yoshida A, et al. The intestine responds to heart failure by enhanced mitochondrial fusion through glucagon-like peptide-1 signalling[J]. Cardiovasc Res, 2019, 115(13):1873-1885.
[14] Drucker DJ. Mechanisms of action and therapeutic application of glucagon-like peptide-1[J]. Cell Metab, 2018, 27(4):740-756.
[15] Liang YL, Khoshouei M, Glukhova A, et al. Phase-plate cryo-EM structure of a biased agonist-bound human GLP-1 receptor-Gs complex[J]. Nature, 2018, 555(7694):121-125.
[16] Meier JJ. GLP-1 receptor agonists for individualized treatment of type 2 diabetes mellitus[J]. Nat Rev Endocrinol, 2012, 8(12):728-742.
[17] Zanotto C, Hansen F, Galland F, et al. Glutamatergic alterations in STZ-induced diabetic rats are reversed by exendin-4[J]. Mol Neurobiol, 2019, 56(5):3538-3551.
[18] Shan Y, Tan S, Lin Y, et al. The glucagon-like peptide-1 receptor agonist reduces inflammation and blood-brain barrier breakdown in an astrocyte-dependent manner in experimental stroke[J]. J Neuroinflammation, 2019, 16(1):242.
[19] Peacock JD, Levay AK, Gillaspie DB, et al. Reduced sox9 function promotes heart valve calcification phenotypes in vivo[J]. Circ Res, 2010, 106(4):712-719.
[20] Thomas PS, Sridurongrit S, Ruiz-Lozano P, et al. Deficient signaling via Alk2(Acvr1)leads to bicuspid aortic valve development[J]. PLoS One 2012, 7(4):e35539.
[21] Fiorotto R, Raizner A, Morell CM, et al. Notch signaling regulates tubular morphogenesis during repair from biliary damage in mice[J]. J Hepatol, 2013, 59(1):124-130.
[22] Zhang X, Xu Y, Chen JM, et al. Huang Qi decoction prevents BDL-induced liver fibrosis through inhibition of notch signaling activation[J]. Am J Chin Med, 2017, 45(1):85-104.
[23] Toshima T, Watanabe T, Narumi T, et al. Therapeutic inhibition of microRNA-34a ameliorates aortic valve calcification via modulation of Notch1-Runx2 signalling[J]. Cardiovasc Res, 2020, 116(5):983-994.
[24] Liu C, Cheng X, Chen J, et al. Suppression of YAP/TAZ-Notch1-NICD axis by bromodomain and extraterminal protein inhibition impairs liver regeneration[J]. Theranostics, 2019, 9(13):3840-3852.
[25] Zanotti S, Canalis E. Interleukin 6 mediates selected effects of Notch in chondrocytes[J]. Osteoarthritis Cartilage, 2013, 21(11):1766-1773.

备注/Memo

备注/Memo:
基金项目:上海交通大学医学院附属第九人民医院基础研究助推计划(JYZZ100)
通信作者:刘艳,E-mail:liuyan_ivy@126.com
更新日期/Last Update: 2021-12-01