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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2021年06期

页码:

360-364

栏目:

基础研究

出版日期:

2021-12-01

文章信息/Info

Title:

Overexpression of c-Ski improves atrial electrical and structural remodeling in canine model of atrial fibrillation

作者:

韩素霞; 王娟; 高苏莉; 盛乐智; 李耀

201200 上海市浦东新区人民医院心内科(韩素霞,高苏莉,盛乐智,李耀); 830001 乌鲁木齐,新疆医科大学第五附属医院心内科(王娟)

Author(s):

HAN Suxia¹; WANG Juan²; GAO Suli¹; SHENG Lezhi¹; LI Yao¹

1.Department of Cardiology, Pudong New Area Peoples' Hospital, Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201200; 2.Department of Cardiology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumchi 830001,

关键词:

心房颤动;  心房重构;  快速心房起搏;  c-Ski

Keywords:

Atrial fibrillation;  Trial structural remodeling;  Rapid atrial pacing;  c-Ski

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2021.06.011

文献标识码:

-

摘要:

目的:分析c-Ski对快速起搏诱导的心房颤动(AF)犬模型心房电重构和结构重构的作用。方法:将18只比格犬随机分为4组:假手术组(n=3)、AF对照组(n=3,仅构建AF模型)、AF-AdNull组(n=6,构建AF模型+注射AdNull腺病毒)及AF-Adc-Ski组(n=6,构建AF模型+注射Adc-Ski腺病毒)。采用电生理实验分析犬AF的诱发率及持续时间。应用苏木精-伊红染色、Masson法染色和天狼猩红染色评价诱导AF后的组织病理学改变。W

Abstract:

Objective:To analyze the effect of c-Ski on atrial electrical and structural remodeling induced by rapid atrial pacing in canine model of atrial fibrillation(AF).Methods:Eighteen beagles were randomly divided into four groups: sham surgery group(n=

参考文献/References

[1] Fan J, Zou L, Cui K, et al. Atrial overexpression of angiotensin-converting enzyme 2 improves the canine rapid atrial pacing-induced structural and electrical remodeling. Fan, ACE2 improves atrial substrate remodeling[J]. Basic Res Cardiol, 2015, 110(4):45.
[2] Beyerbach DM, Zipes DP. Mortality as an endpoint in atrial fibrillation[J]. Heart Rhythm, 2004, 1(2 Suppl):B8-B19.
[3] Everett TH, Li H, Mangrum JM, et al. Electrical, morphological, and ultrastructural remodeling and reverse remodeling in a canine model of chronic atrial fibrillation[J]. Circulation, 2000, 102(12):1454-1460.
[4] Kiryu M, Niwano S, Niwano H, et al. Angiotensin Ⅱ-mediated up-regulation of connective tissue growth factor promotes atrial tissue fibrosis in the canine atrial fibrillation model[J]. Europace, 2012, 14(8):1206-1214.
[5] Li P, Liu P, Peng Y, et al. The ERK/CREB pathway is involved in the c-Ski expression induced by low TGF-β1 concentrations during primary fibroblast proliferation[J]. Cell Cycle, 2018, 17(11):1319-1328.
[6] Ling J, Cai Z, Jin W, et al. Silencing of c-Ski augments TGF-b1-induced epithelial-mesenchymal transition in cardiomyocyte H9C2 cells[J]. Cardiol J, 2019, 26(1):66-76.
[7] Zhang C, Zhang Y, Zhu H, et al. MiR-34a/miR-93 target c-Ski to modulate the proliferaton of rat cardiac fibroblasts and extracellular matrix deposition in vivo and in vitro[J]. Cell Signal, 2018, 46:145-153.
[8] Wang J, He W, Xu X, et al. The mechanism of TGF-β/miR-155/c-Ski regulates endothelial-mesenchymal transition in human coronary artery endothelial cells[J]. Biosci Rep, 2017, 37(4):BSR20160603.
[9] Wang J, Guo L, Shen D, et al. The role of c-Ski in regulation of TGFβ-induced human cardiac fibroblast proliferation and ECM protein expression[J]. J Cell Biochem, 2017, 118(7):1911-1920.
[10] Nakashima H, Kumagai K. Reverse-remodeling effects of angiotensin Ⅱ type 1 receptor blocker in a canine atrial fibrillation model[J]. Circ J, 2007, 71(12):1977-1982.
[11] Zhao Z, Li R,Wang X, et al.Suppression of experimental atrial fibrillation in a canine model of rapid atrial pacing by the phosphodiesterase 3 inhibitor cilostazol[J]. J Electrocardiol, 2020, 60:151-158.
[12] Lu YY, Chen YC, Kao YH, et al. Extracellular matrix of collagen modulates arrhythmogenic activity of pulmonary veins through p38 MAPK activation[J]. J Mol Cell Cardiol, 2013, 59:159-166.

备注/Memo

备注/Memo:

基金项目:国家自然科学基金(81660044); 浦东新区卫健委领先人才(PWRI2017-06); 浦东新区科技发展基金(PKJ2017-Y35)
通信作者:韩素霞,E-mail:lulu57399@163.com

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更新日期/Last Update: 2021-12-01