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[1]何炯红 邓娜 杨龙 唐倩 夏桂玲 杨英 黄勇淇 赵义冬.Crim1过表达抑制肥大乳鼠心室肌细胞瞬时外向钾电流改变[J].国际心血管病杂志,2019,06:346-350.
　HE Jionghong,DENG Na,YANG Long,et al.Overexpression of Crim1 inhibits the change of transient outward potassium current in neonatal rat hypertrophic ventricular myocytes[J].International Journal of Cardiovascular Disease,2019,06:346-350.
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Crim1过表达抑制肥大乳鼠心室肌细胞瞬时外向钾电流改变(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2019年06期
页码:
346-350
栏目:
基础研究
出版日期:
2019-12-31

文章信息/Info

Title:
Overexpression of Crim1 inhibits the change of transient outward potassium current in neonatal rat hypertrophic ventricular myocytes
作者:
何炯红 邓娜 杨龙 唐倩 夏桂玲 杨英 黄勇淇 赵义冬
550002 贵阳, 贵州省人民医院心内科(何炯红,杨龙,夏桂玲,杨英,黄勇淇,赵义冬); 550025 贵阳, 贵州医科大学附属人民医院心内科(邓娜,唐倩)
Author(s):
HE Jionghong1;  DENG Na2;  YANG Long1;  TANG Qian2;  XIA Guiling1;  YANG Ying1;  HUANG Yongqi1;  ZHAO Yidong1
1.Department of Cardiology, Guizhou Provincial People's Hospital, Guizhou 550002; 2. Department of Cardiology, People's Hospital of Guizhou Medical University,Guizhou 550025, China
关键词:
心室肥大;  半胱氨酸丰富跨膜成骨蛋白调控因子;  瞬时外向钾电流;  离子通道
Keywords:
Ventricular hypertrophy;  Cysteine-rich motor neuron 1;  Transient outward potassium current;  Ion channel
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2019.06.007
文献标识码:
-
摘要:
目的:探讨半胱氨酸丰富跨膜成骨蛋白调控因子(Crim1)对肥大乳鼠心室肌细胞瞬时外向钾电流(Ito)的调控作用。方法:将1 d龄SD乳鼠心室肌细胞培养48 h后分组干预。重组腺病毒空载体(Ad-null)组给予Ad-null感染细胞32 h。重组腺病毒空载体+苯肾上腺素(Ad-null+PE)组予Ad-null感染细胞8 h后,再予苯肾上腺素(PE)干预24 h。Crim1过表达重组腺病毒+苯肾上腺素(Ad-Crim1+PE)组给予携带Crim1基因的重组腺病毒感染细胞8 h后,再予PE干预24 h。结晶紫染色培养细胞,ImageJ软件计算细胞表面积。全细胞膜片钳技术检测Ito,计算电流密度。结果:PE干预诱导心室肌细胞肥大,减小Ito电流密度; 该效应可被过表达Crim1所抑制。结论:过表达Crim1可抑制PE诱导的心室肌细胞肥大及Ito改变。
Abstract:
Objective:To investigate the regulation of cysteine-rich motor neuron 1(Crim1)on transient outward potassium current(Ito)in neonatal rat hypertrophic ventricular myocytes.Methods:After cultured for 24 hours, the ventricular myocytes of 1-day old Sprague-Dawley rats were divided into three groups according to different intervention. Null adenoviral vectors(Ad-null)were used to infect the cells in Ad-null group for 32 hours. The cells were infected with empty adenovirus vector for eight hours, then were stimulated with phenylephrine(PE)for 24 hours in Ad-null-PE group. The cells were infected with Crim1-expressing recombinant adenovirus(Ad-Crim1)for eight hours, then were stimulated with phenylephrine for 24 hours in Ad-Crim1-PE group. The cell surface area was evaluated using ImageJ software. Ito was detected by whole-cell patch clamp technique and current density was calculated.Results:PE inducesd ventricular myocyte hypertrophy and reduced Ito current density, which was inhibited by overexpression of Crim1.Conclusions:Overexpression of Crim1 inhibits PE-induced ventricular myocyte hypertrophy and Ito changes.

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备注/Memo

备注/Memo:
基金项目:贵州省科技计划项目(黔科合基础[2019]1205号); 贵州省科学技术厅临床研究中心项目(黔科合平台人才[(2017)5405])
作者单位:550002 贵阳, 贵州省人民医院心内科(何炯红,杨龙,夏桂玲,杨英,黄勇淇,赵义冬); 550025 贵阳, 贵州医科大学附属人民医院心内科(邓娜,唐倩)
通信作者:杨龙,Email:yanglong1001@163.com
更新日期/Last Update: 2019-12-27