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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2019年06期

页码:

341-345

栏目:

基础研究

出版日期:

2019-12-31

文章信息/Info

Title:

Protective effect and mechanism of resveratrol on doxorubicin-induced cardiomyocyte apoptosis

作者:

俞健颖 潘建安 张俊峰 王长谦 顾俊

200011 上海交通大学医学院附属第九人民医院心血管内科

Author(s):

YU Jianying;  PAN Jianan;  ZHANG Junfeng;  WANG Changqian;  GU Jun

Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

关键词:

阿霉素;  心肌细胞;  凋亡;  E2F转录因子1;  白藜芦醇

Keywords:

Doxorubicin;  Cardiomyocyte;  Apoptosis;  E2F transcription factor 1;  Resveratrol

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2019.06.006

文献标识码:

-

摘要:

目的:探讨白藜芦醇(RSV)对阿霉素诱导的心肌细胞凋亡的保护作用及对E2F转录因子1(E2F1)/腺苷酸活化蛋白激酶α2(AMPKα2)信号通路的影响。方法:用小干扰RNA siE2F1转染H9c2心肌细胞,将H9c2细胞分为对照组、阿霉素组、siE2F1组、阿霉素+siE2F1组、RSV组、阿霉素+RSV组,阿霉素终浓度为1 μmol/L,RSV终浓度为20 μmol/L。各组干预24 h后,检测E2F1、AMPKα2和胱天蛋白酶-3剪切体(cleaved caspase-3)的蛋白表达水平及心肌细胞凋亡率。结果:与对照组相比,阿霉素组E2F1、AMPKα2和cleaved caspase-3的蛋白表达水平和心肌细胞凋亡率均显著增加(P均<0.001)。细胞转染后,siE2F1显著抑制E2F1的表达。与阿霉素组相比,阿霉素+siE2F1组E2F1、AMPKα2和cleaved caspase-3的蛋白表达水平及心肌细胞凋亡率显著降低(P均<0.001)。RSV干预能够部分逆转阿霉素诱导的心肌细胞凋亡(P<0.001),与阿霉素组相比,阿霉素+RSV组E2F1、AMPKα2和cleaved caspase-3的蛋白表达水平显著降低(P均<0.001)。结论:RSV可减轻阿霉素诱导的心肌细胞凋亡,其机制可能与抑制E2F1/AMPKα2通路的激活有关。

Abstract:

Objective:To investigate the effects of resveratrol(RSV)on doxorubicin(DOX)-induced cardiomyocyte apoptosis and E2F transcription factor 1(E2F1)/AMP-activated protein kinase α2(AMPKα2)signaling pathway.Methods:A small interfering RNA siE2F1 was used to transfect the rat cardiomyocyte cell line(H9c2). H9c2 cells were divided into six groups: control group, DOX group, siE2F1 group, DOX+siE2F1 group, RSV group and DOX+RSV group. The final concentration of DOX and RSV were 1 μmol/L and 20 μmol/L, respectively. Expression of E2F1, AMPKα2, cleaved caspase-3 and the apoptosis rate of cardiomyocytes were detected at 24 hours after intervention in each group.Results:Compared with the control group, the expression of E2F1, AMPKα2, cleaved caspase-3 and the apoptosis rate of cardiomyocytes significantly increased in the DOX group(P all<0.001). After transfection, siE2F1 significantly inhibited the expression of E2F1. Compared with the DOX group, the expression of E2F1, AMPKα2, cleaved caspase-3 and the apoptosis rate of cardiomyocytes in DOX+siE2F1 group significantly decreased(P all<0.001). RSV partially reversed the apoptosis of cardiomyocytes induced by DOX(P<0.001). The expression of E2F1, AMPKα2 and cleaved caspase-3 significantly decreased in RSV+DOX group compared with the DOX group(P all<0.001).Conclusions:RSV can attenuate DOX-induced cardiomyocyte apoptosis, and its mechanism may be related to the inhibition of E2F1/AMPKα2 pathway activation.

参考文献/References

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备注/Memo

备注/Memo:

基金项目:国家自然科学基金(81670293)
作者单位:200011 上海交通大学医学院附属第九人民医院心血管内科
通信作者:顾俊,Email:gjforsub@163.com

常用功能

更新日期/Last Update: 2019-12-27