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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2018年05期

页码:

281-284,303

栏目:

基础研究

出版日期:

2019-02-01

文章信息/Info

Title:

The role of HSF1 in cardiac amyloidosis in mice

作者:

钱俊乔 霍佳 钱金泽 王艳军 李瑞琛 段惠军 杜春阳

Author(s):

QIAN Junqiao¹;  HUO Jia²;  QIAN Jinze³;  WANG Yanjun³;  LI Ruichen³;  DUAN Huijun³;  DU Chunyang³

1. Department of Oral Surgery, Oral hospital of Hebei Province, Hebei 050000; 2. Department of Osteopathy, The Third Hospital of Hebei Province, Hebei 050000; 3. Department of Pathology/the Key Laboratory of Kidney Diseases of Hebei Province, Hebei Medic

关键词:

热休克转录因子;  心脏淀粉样变;  热休克蛋白

Keywords:

Heat shock factor;  Cardiac amyloidosis;  Heat shock protein

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2018.05.007

文献标识码:

-

摘要:

目的:探讨热休克转录因子1(HSF1)在心脏淀粉样变疾病中的作用机制。方法:将30只HSF1敲除小鼠随机分为3个实验组,HSF1敲除型空白对照组、敲除型淀粉样变模型组及替普瑞酮治疗组; 将20只野生型小鼠随机分为2个实验组,野生型空白对照组、野生型淀粉样变模型组,每组均为10只。进行淀粉样变诱导实验构建淀粉样变疾病模型。模型构建2个月后处死小鼠,通过刚果红染色、免疫组织化学染色、分子生物学检测,比较各个实验组心脏组织中热休克蛋白及淀粉样变沉积情况,以及肝脏、脾脏、胃、肠舌、皮肤组织中的淀粉样变沉积情况。

Abstract:

Objective:To probe the role of heat shock factor 1(HSF1)in cardiac amyloidosis in mice model.Methods:Thirty female HSF1^-/- mice were divided into 3 random groups(negative control of HSF1^-/-, AA induction of HSF1^-/-, AA ind

参考文献/References

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备注/Memo

备注/Memo:

基金项目:国家自然科学基金(81300606); 河北省高等学校科学技术研究项目(BJ2014042); 高等学校博士科学点专项
科研基金(2012323120012); 河北省卫生厅医学科学研究课题计划项目(ZD20140355)
作者单位:050017 石家庄,河北医科大学病理学教研室,河北省肾脏病重点实验室(钱金泽,段惠军,杜春阳); 050000

常用功能

更新日期/Last Update: 2018-09-30