|本期目录/Table of Contents|

[1]杨英,夏桂玲,杨龙,等.血管紧张素Ⅱ受体1型-钙调神经磷酸酶信号通路对乳鼠肥大心室肌细胞L型钙离子流的调控作用[J].国际心血管病杂志,2021,05:296-300.
　YANG Ying,XIA Guiling,YANG Long,et al.Regulation of AT1R-calcineurin signaling pathway on L-type calcium current of hypertrophic ventricular myocytes from neonatal rats[J].International Journal of Cardiovascular Disease,2021,05:296-300.
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血管紧张素Ⅱ受体1型-钙调神经磷酸酶信号通路对乳鼠肥大心室肌细胞L型钙离子流的调控作用(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2021年05期
页码:
296-300
栏目:
基础研究
出版日期:
2021-10-01

文章信息/Info

Title:
Regulation of AT1R-calcineurin signaling pathway on L-type calcium current of hypertrophic ventricular myocytes from neonatal rats
作者:
杨英;夏桂玲;杨龙;何炯红;霍照美;郭楚娴
550002 贵阳,贵州省人民医院心内科
Author(s):
YANG Ying;  XIA Guiling;  YANG Long;  HE Jionghong;  HUO Zhaomei;  GUO Chuxian
Department of Cardiology, Guizhou Provincial People’s Hospital, Guiyang 550002, China
关键词:
心室肌细胞;  钙电流;  动作电位;  全细胞膜片钳技术
Keywords:
Ventricular myocyte;  Calcium current;  Action potential;  Whole-cell patch clamp technique
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2021.05.011
文献标识码:
-
摘要:
目的:探讨血管紧张素Ⅱ受体1型(AT1R)-钙调神经磷酸酶(CaN)信号通路对乳鼠肥大心室肌细胞L型钙离子通道电流(ICa-L)和动作电位时程(APD)的调控作用。方法:分离获得1 d龄SD乳鼠心室肌细胞,分为4组,对照组不予干预; 苯肾上腺素(PE)组常规培养1 h后加入PE(终浓度0.1 mmol/L)干预24 h; 氯沙坦(Los)+PE组先予Los(终浓度1 μmol/L)干预1 h,再予PE干预24 h; 环孢素A(CsA)+PE组先予CsA(终浓度0.25 μg/mL)干预1 h,再予PE干预24 h。实时荧光定量聚合酶链反应检测肌球蛋白重链β(β-MHC)的mRNA表达水平。Western blot检测CaN A亚基β亚单位(CaNAβ)蛋白表达水平。全细胞膜片钳技术检测细胞膜ICa-L和单细胞APD。结果:PE组β-MHC的mRNA表达水平和CaNAβ的蛋白表达水平均高于对照组; ICa-L电流密度峰值绝对值[(-18.23±1.44)pA/pF对(-10.78±1.87)pA/pF]、50%APD[(25.7±2.4)ms对(15.3±3.0)ms]和90%APD[(197.6±10.6)ms对(91.8±21.3)ms]均高于对照组(P均<0.05)。Los+PE组β-MHC的mRNA表达水平和CaNAβ的蛋白表达水平均低于PE组; ICa-L电流密度峰值绝对值[(-14.02±1.51)pA/pF]、50% APD [(20.4±2.9)ms]和90% APD [(128.7±18.5)ms]均低于PE组(P均<0.05)。CsA+PE组β-MHC的mRNA表达水平和CaNAβ的蛋白表达水平均低于PE组; ICa-L电流密度峰值绝对值[(-12.19±1.09)pA/pF]、50% APD [(22.7±1.9)ms]和90% APD [(121.7±15.0)ms]均低于PE组(P均<0.05)。结论:AT1R-CaN信号通路参与乳鼠肥大心室肌细胞ICa-L和APD的调控。
Abstract:
Objective:To investigate the role of angiotensin Ⅱ receptor 1 type(AT1R)-calcineurin(CaN)signaling pathway in regulating L-type Ca2+ channel current(ICa-L)and action potential duration(APD)of hypertrophic ventricular myocytes from ne

参考文献/References

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备注/Memo

备注/Memo:
基金项目:贵州省科技计划项目(黔科合基础[2019]1205); 贵州省科技平台及人才团队计划(黔科合平台人才[(2017)5405])
通信作者:杨龙,E-mail:yanglong1001@163.com
更新日期/Last Update: 2021-10-01