|本期目录/Table of Contents|

[1]刘强,韩晨俊,吴晓云,等.长期高脂饮食对小鼠心肌组织自噬、凋亡及炎性反应的影响[J].国际心血管病杂志,2021,05:301-305.
　LIU Qiang,HAN Chenjun,WU Xiaoyun,et al.Effects of long-term high-fat diet on autophagy, apoptosis and inflammation in myocardium of mice[J].International Journal of Cardiovascular Disease,2021,05:301-305.
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长期高脂饮食对小鼠心肌组织自噬、凋亡及炎性反应的影响(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2021年05期
页码:
301-305
栏目:
基础研究
出版日期:
2021-10-01

文章信息/Info

Title:
Effects of long-term high-fat diet on autophagy, apoptosis and inflammation in myocardium of mice
作者:
刘强;韩晨俊;吴晓云;臧旺福;周健
200070 上海,同济大学附属第十人民医院心胸外科
Author(s):
LIU Qiang;  HAN Chenjun;  WU Xiaoyun;  ZANG Wangfu;  ZHOU Jian
Department of Cardiac Surgery, Shanghai Tenth People’s Hospital, Shanghai 200072, China
关键词:
自噬;  凋亡;  炎性反应;  高脂饮食;  心肌组织
Keywords:
Autophagy;  Apoptosis;  Inflammation;  High-fat diet;  Myocardium
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2021.05.012
文献标识码:
-
摘要:
目的:研究长期高脂饮食对小鼠心肌组织自噬、凋亡及炎性反应的影响。方法:6周龄雄性C57BL/6小鼠随机分为对照组和高脂喂养组,饲养至24周时,称量体质量,取小鼠心脏及附睾周围脂肪组织并称重,计算心脏质量/体质量比值; Western blot和实时荧光定量聚合酶链反应检测小鼠心肌组织自噬、心肌凋亡及炎性反应相关基因的mRNA和蛋白表达水平。结果:与对照组相比,高脂喂养组体质量、内脏脂肪和心脏组织质量均明显增加,心脏质量/体质量的比值也明显增加(P均<0.05); 小鼠心肌组织自噬标志基因LC3Ⅱ的mRNA和蛋白表达明显降低,自噬核心基因Becn1的mRNA和蛋白表达水平也明显降低(P均<0.05); 凋亡相关基因Bax和Bad的mRNA表达水平均明显升高,凋亡标志蛋白裂解型胱天蛋白酶-3(c-caspase-3)和裂解型多聚腺苷二磷酸核糖聚合酶(c-PARP)的蛋白表达水平均明显升高(P均<0.05); 炎性基因NLRP3、白细胞介素(IL)-1β和胱天蛋白酶-1(caspase-1)的mRNA表达水平均明显增加(P均<0.05)。结论:长期高脂饮食抑制小鼠心肌组织自噬,增加心肌细胞凋亡和炎性反应相关基因的表达。
Abstract:
Objective:To study the effect of long-term high-fat diet on autophagy, apoptosis and inflammation of myocardium in mice.Methods:Six-week-old male C57BL/6 mice were randomly divided into the control group and high-fat feeding group. All the mice were weigh

参考文献/References

[1] Lavie CJ, Alpert M, Arena R, et al. Impact of obesity and the obesity paradox on prevalence and prognosis in heart failure[J]. JACC Heart Fail, 2013, 1(2):93-102.
[2] Wang ZV, Ferdous A, Hill JA. Cardiomyocyte autophagy:metabolic profit and loss[J]. Heart Fail Rev, 2013, 18(5):585-594.
[3] Klionsky DJ, Abdalla FC, Abeliovich H, et al. Guidelines for the use and interpretation of assays for monitoring autophagy[J]. Autophagy, 2012, 8(4):445-544.
[4] De Meyer GR, Martinet W. Autophagy in the cardiovascular system[J]. Biochim Biophys Acta, 2009, 1793(9):1485-1495.
[5] Tanaka Y, Guhde G, Suter A, et al. Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice[J]. Nature, 2000, 406(6798):902-906.
[6] Rifki OF, Hill JA. Cardiac autophagy: good with the bad[J]. J Cardiovasc Pharmacol, 2012, 60(3):248-252.
[7] Li S, Liu C, Gu L, et al. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36[J]. Open Biol, 2016, 6(8):160177.
[8] Taube A, Schlich R, Sell H, et al. Inflammation and metabolic dysfunction: links to cardiovascular diseases[J]. Am J Physiol Heart Circ Physiol, 2012, 302(11):H2148-H2165.
[9] Kim NH, Kang PM. Apoptosis in cardiovascular diseases: mechanism and clinical implications[J]. Korean Circ J, 2010, 40(7):299-305.
[10] Kang R, Zeh HJ, Lotze MT, et al. The beclin 1 network regulates autophagy and apoptosis[J]. Cell Death Differ, 2011, 18(4):571-580.
[11] Zhong ZS, Karin M. Autophagy, NLRP3 inflammasome and auto-inflammatory/immune diseases[J]. Clin Exp Rheumatol, 2016, 34(4 Suppl 98):12-16.
[12] Hsu HC, Chen CY, Lee BC, et al. High-fat diet induces cardiomyocyte apoptosis via the inhibition of autophagy[J]. Eur J Nutr, 2016, 55(7):2245-2254.
[13] Zeng H, Vaka VR, He X, et al. High-fat diet induces cardiac remodelling and dysfunction: assessment of the role played by SIRT3 loss[J]. J Cell Mol Med, 2015, 19(8):1847-1856.
[14] Zhu H, Rothermel BA, Hill JA. Autophagy in load-induced heart disease[J]. Methods Enzymol, 2009, 453:343-363.
[15] Lancel S, Montaigne D, Marechal X, et al. Carbon monoxide improves cardiac function and mitochondrial population quality in a mouse model of metabolic syndrome[J]. PLoS One, 2012, 7(8):e41836.
[16] Wang Z, Li L, Zhao H, et al. Chronic high fat diet induces cardiac hypertrophy and fibrosis in mice[J]. Metabolism, 2015, 64(8):917-925.
[17] Wencker D, Chandra M, Nguyen K, et al. A mechanistic role for cardiac myocyte apoptosis in heart failure[J]. J Clin Invest, 2003, 111(10):1497-1504.
[18] Ciechomska IA, Goemans GC, Skepper JN, et al. Bcl-2 complexed with Beclin-1 maintains full anti-apoptotic function[J]. Oncogene, 2009, 28(21):2128-2141.
[19] Djavaheri-Mergny M, Maiuri MC, Kroemer G. Cross talk between apoptosis and autophagy by caspase-mediated cleavage of Beclin 1[J]. Oncogene, 2010, 29(12):1717-1719.
[20] Saitoh T, Akira S. Regulation of inflammasomes by autophagy[J]. J Allergy Clin Immunol, 2016, 138(1):28-36.

备注/Memo

备注/Memo:
基金项目:上海市卫生和计划生育委员会面上项目(201440476); 上海市自然科学基金(18ZR1435800)
通信作者:周健,E-mail:drjose@163.com
更新日期/Last Update: 2021-10-01