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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2018年04期

页码:

219-224

栏目:

基础研究

出版日期:

2018-07-25

文章信息/Info

Title:

Mechanism of dehydroepiandrosterone resisting atherosclerosis by inhibiting the expression of MIP-1α induced by high lipid

作者:

周颖 李桃 陈艳昕 肖芳 赵敏

Author(s):

ZHOU Ying;  LI Tao;  CHEN Yanxin;  XIAO Fang;  ZHAO Min.

Department of Pathology, The First Hospital of Qinhuangdao, Hebei 066000, China

关键词:

动脉粥样硬化;  脱氢表雄酮;  细胞色素P450芳香酶基因;  巨噬细胞炎性蛋白-1α;  人脐静脉内皮细胞

Keywords:

Artherosclerosis;  Dehydroepiandrosterone;  CYP19;  Macrophage inflammatory protein-1α;  Human umbilical venous endothelial cells

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2018.04.008

文献标识码:

-

摘要:

目的:探讨脱氢表雄酮(DHEA)是否通过抑制高脂诱导的巨噬细胞炎性蛋白-1α(MIP-1α)的表达发挥抗动脉粥样硬化(AS)作用,以及细胞色素P450芳香酶基因(CYP19)对DHEA的作用。方法: 氧化低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVEC),给予DHEA、全反式维甲酸(ATRA)干预; pcDNA3.1-CYP19-GFP真核表达质粒及pcDNA3.1-GFP空质粒分别转染HUVEC,给予ox-LDL诱导及DHEA干预。实时荧光定量PCR和细胞酶联免疫吸附法检测各组HUVEC及

Abstract:

Objective:To investigate whether dehydroepiandrosterone(DHEA)can resist atherosclerosis by inhibiting the expression of macrophage inflammatory protein-1α(MIP-1α)induced by high lipid, and the effect of cytochrome P450 aromatase gene(CYP19)on DHEA.Method

参考文献/References

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备注/Memo

备注/Memo:

基金项目:秦皇岛市科技支撑计划项目(201703A098)
作者单位:066000 秦皇岛市第一医院病理科
通信作者:赵敏,Email:zhouying8042254@163.com

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更新日期/Last Update: 2018-07-30