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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2024年02期

页码:

109-113

栏目:

基础研究

出版日期:

2024-03-25

文章信息/Info

Title:

HMGB1 induces myocardial fibrosis by regulating autophagy factor P62

作者:

聂雅琴; 乌日娜; 金慧; 张广平; 李永明; 智利; 薛仕煊; 娜日格乐

014040 包头医学院中心临床医学院（聂雅琴）；014040 包头市中心医院心血管内科（乌日娜，金慧，张广平， 李永明， 智利， 薛仕煊， 娜日格乐）

Author(s):

NIE Yaqin¹;  WU Rina²;  JIN Hui²;  ZHANG Guangping²;  LI Yongming²;  ZHI Li²;  XUE Shixuan²;  NARI Gele²

1.Baotou Medical College Center Clinical Medical College, Baotou 014040; 2.Cardiovascular Department of Baotou Central Hospital, Baotou? 014040, China

关键词:

高迁移率族蛋白1 ; AC16细胞; 自噬; 心肌纤维化

Keywords:

High mobility group protein;  AC16 cells;  Autophagy;  Myocardial fibrosis

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2024.02.012

文献标识码:

-

摘要:

目的：探究高迁移率族蛋白1（HMGB1）通过调控自噬活性对心肌纤维化的影响及作用机制。方法：将人心肌细胞AC16培养于DMEM培养基中，用不同浓度的HMGB1（0、4、20、100μg/L）干预AC16细胞6h，免疫荧光染色检测心脏成纤维细胞活化的标志性蛋白α平滑肌肌动蛋白（α-SMA）的表达水平，Westernblot法检测心肌自噬相关蛋白哺乳动物雷帕霉素靶蛋白（mTOR）、磷酸化mTOR（p-mTOR）、微管相关蛋白1轻链3（LC3）、磷酸肌醇-3-激酶3（PIK3C3）、可溶性及不可溶性P62、α-

Abstract:

Objective: To explore the effect of high mobility group box protein 1 (HMGB1) on myocardial fibrosis by regulating autophagy activity of human cardiomyocytes (AC16) and its possible mechanism.? Methods：AC16 cells were cultured in DMEM medium and treated w

参考文献/References

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备注/Memo

备注/Memo:

基金项目：内蒙古医科大学科技百万工程联合项目[YKD2020KJBW(LH)065]
通信作者：乌日娜， E-mail：46505898@qq.com

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更新日期/Last Update: 2024-03-25