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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2023年03期

页码:

161-164

栏目:

基础研究

出版日期:

2023-05-30

文章信息/Info

Title:

Effects of miRNA-27b on expression of apoptosis related factors in hypertrophic cardiomyocytes induced by angiotensin Ⅱ?

作者:

陈运起; 吴钟伟; 李堪董; 赵圣吉

571700 儋州，海南西部中心医院心血管内科

Author(s):

CHEN Yunqi; WU Zhongwei; LI Kandong; ZHAO Shengji?

Department of Cardiology, Hainan West Central Hospital, Danzhou? 571700, China

关键词:

心肌肥大; 血管紧张素Ⅱ; miRNA-27b ; 细胞凋亡

Keywords:

Cardiomyocyte hypertrophy;  Angiotensin Ⅱ;  MiRNA-27b;  Apoptosis

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2023.03.009

文献标识码:

-

摘要:

目的：观察微小RNA-27b（miR-27b）对血管紧张素Ⅱ（AngⅡ）诱导肥大心肌细胞凋亡率及凋亡相关蛋白表达的影响。方法：将H9c2小鼠心肌细胞分为4组，分别为正常对照组、AngⅡ诱导组、miR-27bmimics组和阴性核苷酸组。各组细胞建模培养后分别检测心肌细胞凋亡率，Bax、Bcl-2蛋白和miR-27b的表达，监测心肌细胞内活性氧（ROS）水平的变化以及总超氧化物歧化酶（SOD）活性。结果：免疫荧光染色可见AngⅡ诱导组心肌细胞均较正常对照组明显肥大，细胞表面积增大，而miR-27bmimics组细胞肥大明显改善（P＜0.05）。与正常对照组比较，AngⅡ诱导组Bax蛋白表达上调，而Bcl-2蛋白和miR-27b表达明显下调（P＜0.05）。与AngⅡ诱导组比较，miR-27bmimics组Bax蛋白表达则减少，而Bcl-2蛋白和miR-27b表达增加（P＜0.05）。与正常对照组相比，AngⅡ诱导组凋亡率显著增加（P＜0.01），而与AngⅡ诱导组比较，miR-27bmimics组凋亡率明显改善（P＜0.05）。AngⅡ诱导组心肌细胞ROS水平较正常对照组上升，而总SOD活性下降，2组间比较差异均有统计学意义（P＜0.05或P＜0.01）。与AngⅡ诱导组比较，miR-27bmimics组ROS水平下降，而总SOD活性增加（P＜0.05）。结论：miR-27b可能通过调控细胞凋亡的基因转录，参与心肌细胞肥大的发生发展。

Abstract:

Objective: To determine the effect of microRNA-27b ( miR-27b ) on apoptosis rate and apoptosis-related protein expression of hypertrophic cardiomyocytes induced by angiotensin Ⅱ（AngⅡ）.? Methods: H9c2 mouse cardiomyocytes were divided into normal control group, AngⅡ-induced group, miR-27b mimics group, and negative nucleotide group. In each group, apoptosis rate of cardiomyocytes, expressions of Bax, Bcl-2 and miR-27b, and changes of ROS level and total SOD activity were assessed after modeling and cell culture.? Results: Immunofluorescence staining showed that myocardial cells were larger and cell surface area was greater in AngⅡ-induced group than those in normal control group. These features of cell hypertrophy induced by AngⅡ was significantly improved in miR-27b mimics group (P＜0.05). Compared with normal control group, AngⅡinduced an up-regulation of Bax protein expression and a down-regulation of Bcl-2 protein expression and miR-27b (P＜0.05), which were reversed in miR-27b mimics group (P＜0.05). Similarly, compared with normal control group,apoptosis rate was increased in AngⅡ-induced group (P＜0.01), which was improved in miR-27b mimics group (P＜0.05). In addition, AngⅡ induced an increase in ROS level and a decrease in total SOD activity in the myocardial cells (P＜0.05 and P＜0.01,respectively). ROS level was lower while SOD activity was higher in miR-27b mimics group than in AngⅡ-induced group (all P＜0.05).? Conclusion: Our results suggest that miR-27b may participate in the development of cardiomyocyte hypertrophy by regulating gene transcription of apoptosis.

参考文献/References

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备注/Memo

备注/Memo:

基金项目：海南省自然科学基金（820RC781）

常用功能

更新日期/Last Update: 2023-05-30