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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2019年06期

页码:

358-362

栏目:

基础研究

出版日期:

2019-12-31

文章信息/Info

Title:

Effects of deferoxamine pretreatment combined with sevoflurane postconditioning on myocardial ischemia-reperfusion injury in diabetic rats

作者:

杨龙 戴晓雯 马宁 吴建江 王江 郑宏

830054 乌鲁木齐,新疆医科大学第一附属医院麻醉科

Author(s):

YANG Long;  DAI Xiaowen;  MA Ning;  WU Jianjiang;  WANG Jiang;  ZHENG Hong

Department of Anesthesiology, First Affiliated Hospital of Xinjiang Medical University, Xinjiang 830054, China

关键词:

糖尿病;  七氟醚后处理;  缺血再灌注损伤;  缺氧诱导因子-1α

Keywords:

Diabetes;  Sevoflurane postconditioning;  Ischemia-reperfusion injury;  Hypoxia-inducible factor-1α

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2019.06.009

文献标识码:

-

摘要:

目的:探讨去铁胺(DFO)预处理联合七氟醚后处理(SPostC)对糖尿病大鼠心肌缺血再灌注(I/R)损伤的影响。方法:采用Langendorff离体灌流装置建立I/R模型,缺血40 min,再灌注2 h。通过给予DFO预处理和(或)SPostC,观察各组再灌注末的心率、左室发展压(LVDP)、左室舒张末期压力(LVEDP)及左室内压最大上升速率(+dp/dtmax)等血流动力学指标的变化,Western blot法测定心肌缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)的表达水平变化,电镜观察心肌线粒体超微结构,TTC染色法测定心肌梗死面积。结果:与对照组相比,其余各组心率、LVDP、+dp/dtmax均明显降低,LVEDP和心肌梗死面积均明显增加,HIF-1α、VEGF表达水平均明显上调(P均<0.05),线粒体结构有不同程度的破坏。与I/R组相比,DFO组、DFO+SPostC组LVEDP均明显降低,心率、LVDP、+dp/dtmax均明显改善,HIF-1α、VEGF表达水平均明显上调,心肌梗死面积均明显减少(P均<0.05),线粒体结构完整度明显改善。与DFO组相比,DFO+SPostC组LVEDP明显降低,心率、LVDP、+dp/dtmax明显改善,HIF-1α、VEGF表达上调,心肌梗死面积减少(P均<0.05),线粒体结构基本完整,略有肿胀。结论:DFO预处理联合SPostC可以减轻糖尿病大鼠心肌I/R损伤。

Abstract:

Objective:To investigate the effects of deferoxamine pretreatment combined with sevoflurane postconditioning on myocardial ischemia-reperfusion injury(I/R)in diabetic rats.Methods:The I/R mode was established by using Langendorff perfusion apparatus and the rats were subjected to ischemia for 40 min followed by reperfusion for two hours. After deferoxamine pretreatment and/or sevoflurane postconditioning, hemodynamic parameters such as heart rate(HR), left ventricular development pressure(LVDP), left ventricular end-diastolic pressure(LVEDP), and maximal rate of increase of left ventricular pressure(+dp/dtmax)were recorded in each group. The expression levels of hypoxia-inducible factor(HIF)-1α and vascular endothelial growth factor(VEGF)were determined by western blot. The ultrastructure of mitochondria was observed by electron microscope. The area of myocardial infarction was determined by TTC staining method.Results:Compared with the control group, HR, LVDP and +dp/dtmax in other groups were significantly decreased, and LVEDP and the area of myocardial infarction were significantly increased(P all <0.05). The expression of HIF-1α and VEGF was up-regulated and mitochondrial structure had different degrees of damage(P all <0.05). Compared with I/R group, in the DFO group and DFO+SPostC group, LVEDP was significantly decreased; HR, LVDP and+dp/dtmax were significantly improved; the expression of HIF-1α and VEGF was up-regulated, and the area of myocardial infarction decreased(P all <0.05). The structure of mitochondria was relatively completed in the DFO group and DFO+SPostC group. As compared with DFO group, LVEDP was significantly decreased and HR, LVDP as well as+dp/dtmax were significantly improved; the expression of HIF-1α and VEGF was up-regulated, and the myocardial infarcttion size decreased in the DFO+SPostC group(P all <0.05). The mitochondria structure was almost complete and slightly swollen in DFO+SPostC group.Conclusions:Deferoxamine pretreatment combined with sevoflurane postconditioning can alleviate myocardial IR injury in diabetic rats.

参考文献/References

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备注/Memo

备注/Memo:

基金项目:新疆维吾尔自治区自然科学基金(2017D01C340)
作者单位:830054 乌鲁木齐,新疆医科大学第一附属医院麻醉科
通信作者:郑宏,Email:xjzhenghong@aliyun.com

常用功能

更新日期/Last Update: 2019-12-27