|本期目录/Table of Contents|

[1]张鹏,毕昌龙,魏萌,等.酰基甘油激酶通过影响PTEN/PI3K/Akt/Gsk3β 信号通路调控血小板活化[J].国际心血管病杂志,2023,01:46-51.
　ZHANG Peng,BI Changlong,WEI Meng,et al.Platelet activation is regulated by acylglycerol kinase via activating PTEN/PI3K/Akt/GSK3β signaling pathway[J].International Journal of Cardiovascular Disease,2023,01:46-51.
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酰基甘油激酶通过影响PTEN/PI3K/Akt/Gsk3β 信号通路调控血小板活化(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2023年01期
页码:
46-51
栏目:
基础研究
出版日期:
2023-01-20

文章信息/Info

Title:
Platelet activation is regulated by acylglycerol kinase via activating PTEN/PI3K/Akt/GSK3β signaling pathway
作者:
张鹏; 毕昌龙; 魏萌; 姜哲轶; 张田田; 张俊峰
200011 上海交通大学医学院附属第九人民医院心血管内科
Author(s):
ZHANG Peng;  BI Changlong;  WEI Meng;  JIANG Zheyi;  ZHANG Tiantian;  ZHANG Junfeng
Department of Cardiology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
关键词:
酰基甘油激酶; 血小板活化; 张力蛋白同源性磷酸酶; 磷脂酰肌醇3- 激酶;  蛋白激酶B ; 糖原合成激酶3β
Keywords:
Acylglycerol kinase;  Platelet activation;  Phosphatase and tensin homolog;  Phosphatidylinositol-3-kinase;  Protein kinase B;  Glycogen synthase kinase 3β
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2023.01.012
文献标识码:
-
摘要:
目的：探究酰基甘油激酶（AGK）在血小板活化中的作用及可能的调控机制。 方法：分别提取野生型小鼠（WT）和AgkG126E 点突变（AGK 激酶活性丧失）小鼠（PM） 的血小板。将等体积、等浓度的WT 或PM 小鼠的血小板放入聚集仪，分别利用3 种刺激 剂（α-Thrombin、ADP、U46619）刺激，检测每种刺激剂作用下相应的血小板聚集水平；每 管聚集曲线达到最大稳定值后，加入等体积的ATP 荧光检测剂，检测相应血小板活化过程中 释放的ATP 水平。分别在静息态WT 或PM 小鼠血小板（不加α-Th
Abstract:
Objective：To explore the role and possible regulatory mechanism of acylglycerol kinase (AGK) during platelet activation. Methods: Platelets were extractedfrom wild-type mice (WT) and Agk G126E point mutant mice (deprivation of AGK activity), respectively.

参考文献/References

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81670316,81970289）
通信作者：张俊峰，E-mail：jfzhang_dr@163.com
更新日期/Last Update: 2023-01-20