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《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:

2022年06期

页码:

367-370

栏目:

基础研究

出版日期:

2022-12-20

文章信息/Info

Title:

Effect of Bmal1 gene overexpression on the degree of atherosclerosis and plaque stability

作者:

朱胜奎; 丁家望; 汪心安; 张再强; 万剑桥

443003 宜昌， 三峡大学第一临床医学院， 宜昌市中心人民医院心内科

Author(s):

ZHU Shengkui;  DING Jingwang;  WANG Xinan;  ZHANG Zaiqing;  WAN Qianqiao

Department of Cardiology, Yichang Central People’s Hospital, First Medical College of Sanxia University, Yichang 443003, China

关键词:

脑肌类芳烃受体核转位样蛋白1 ; 动脉粥样硬化; 基质金属蛋白酶

Keywords:

Bmal1;  Atherosclerosis;  Matrix metalloproteinases

分类号:

-

DOI:

10.3969/j.issn.1673-6583.2022.06.014

文献标识码:

-

摘要:

目的：探讨昼夜节律基因脑肌类芳烃受体核转位样蛋白1（Bmal1）过表达对 小鼠动脉粥样硬化斑块病变程度及斑块稳定性的影响。 方法：12 只血管平滑肌细胞特异 性Bmal1 转基因的SM-Bmal1-TG/ApoE-/- 小鼠和12 只ApoE-/- 小鼠均予以高脂饮食，行颈 总动脉CAST 套管结扎术使小鼠形成动脉粥样硬化斑块。喂养11 周后处死动物，取出颈总 动脉血管，行苏木精- 伊红染色检测小鼠主动脉粥样硬化程度及斑块面积，Western blot 法 检测及基质金属蛋白酶（MMP）-2、MMP-9 的蛋白表达水平。 结果：与ApoE-/- 组相比， SM-Bmal1-TG/ApoE-/- 组三酰甘油（TC）、总胆固醇（TG）、低密度脂蛋白胆固醇（LDL-C） 水平均明显降低（P 均＜0.05），2 组高密度脂蛋白胆固醇（HDL-C）水平的差异无统计学意义。 SM-Bmal1-TG/ApoE-/- 组小鼠颈总动脉斑块减少约25%，内膜增厚不明显。与ApoE-/- 组相比， SM-Bmal1-TG/ApoE-/- 组MMP-2、MMP-9 的蛋白表达水平均明显降低（P 均＜0.05）。 结 论：Bmal1 可抑制动脉粥样硬化斑块病变程度，下调主动脉粥样斑块内MMP-2、MMP-9 水平， 具有抗动脉粥样硬化的作用。

Abstract:

Objective: To investigate the effect of circadian rhythm gene BMAL1 on the degree of atherosclerosis and plaque stability. Methods: BMAL1 overexpression mice (SMBmal1- TG/ApoE-/-) and ApoE-/- mice were treated with high-fat diet, and atherosclerotic plaqu

参考文献/References

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备注/Memo

备注/Memo:

基金项目：国家自然科学基金（81770456）
通信作者：丁家望， E-mail：dingjiawang@qq.com

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更新日期/Last Update: 2022-12-20