|本期目录/Table of Contents|

[1]韩晓宇,黄佑群,钟宏.亚精胺对心肌梗死大鼠心肌线粒体功能及细胞凋亡的影响[J].国际心血管病杂志,2021,02:106-111.
　HAN Xiaoyu,HUANG Youqun,ZHONG Hong..Effects of spermidine on cardiomyocyte and mitochondrial function apoptosis in rats with myocardial infarction[J].International Journal of Cardiovascular Disease,2021,02:106-111.
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亚精胺对心肌梗死大鼠心肌线粒体功能及细胞凋亡的影响(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2021年02期
页码:
106-111
栏目:
基础研究
出版日期:
2021-03-20

文章信息/Info

Title:
Effects of spermidine on cardiomyocyte and mitochondrial function apoptosis in rats with myocardial infarction
作者:
韩晓宇; 黄佑群; 钟宏
610017 成都市第二人民医院全科老年医学科(韩晓宇,钟宏); 611137 成都中医药大学肾病内科(黄佑群)
Author(s):
HAN Xiaoyu1;  HUANG Youqun2;  ZHONG Hong1.
1.Department of General Geriatrics, Chengdu Second People's Hospital, Chengdu 610017, China; 2.Department of Nephrology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
关键词:
心肌梗死;  心肌细胞凋亡;  亚精胺
Keywords:
Myocardial infarction;  Cardiomyocyte apoptosis;  Spermidine
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2021.02.011
文献标识码:
-
摘要:
目的:探讨亚精胺对心肌梗死大鼠心肌线粒体功能及细胞凋亡的影响。方法:选取健康SD大鼠32只,随机分为假手术组、模型组、模型+阿司匹林组、模型+亚精胺组,每组8只,利用在体结扎冠状动脉前降支法建立急性心肌梗死模型。造模成功后假手术组和模型组给予无菌生理盐水(2 mL)灌胃,模型+阿司匹林组以等体积阿司匹林(20 mg/kg)灌胃,模型+亚精胺组以等体积亚精胺(5 mmol/L)灌胃,连续4周。TTC染色、HE染色观察心肌组织梗死面积和病理学改变; 透射电镜观察心肌线粒体形态; 原位缺口末端标记(TUNEL)法检测心肌组织细胞凋亡水平; 实时荧光定量PCR(qRT-PCR)检测Bax、Bcl-2、胱天蛋白酶-3(caspase-3)的mRNA表达水平; Western blot检测Bcl-2、Bax、caspase-3、细胞色素C(cytochrome C)的蛋白表达水平。结果:与假手术组比较,模型组大鼠心肌组织梗死区面积增加,心肌组织细胞凋亡明显增加,心肌组织Bax、caspase-3、cytochrome C表达水平明显升高,Bcl-2表达水平明显降低(P均<0.01); 与模型组比较,模型+亚精胺组大鼠心肌组织梗死区面积减少,心肌组织细胞凋亡及Bax、caspase-3、cytochrome C表达水平均明显减少(P<均0.05); 与模型+阿司匹林组比较,模型+亚精胺组心肌组织细胞凋亡明显降低(P<0.01),两组心肌组织Bax、caspase-3、Bcl-2、cytochrome C表达水平的差异无统计学意义。结论:亚精胺可改善心肌线粒体功能,抑制细胞凋亡,改善心肌梗死。
Abstract:
Objective:To investigate the effect of spermidine on cardiomyocyte apoptosis and mitochondrial function in rats with myocardial infarction.Methods:A total of 32 healthy SD rats were randomly divided into sham operation group, model group, model+aspirin gr

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备注/Memo

备注/Memo:
通信作者:钟宏,E-mail:772579491@qq.com
更新日期/Last Update: 2021-03-20