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[1]吕瑾 杨胜祥 华晓芳 刘长召.黑芥子苷通过p38/JNK MAPK信号通路对阿霉素心脏毒性的保护作用[J].国际心血管病杂志,2020,01:48-51.
　Lv Jin,YANG Shengxiang,HUA Xiaofang,et al.Sinigrin protects against doxorubicin-induced cardiotoxicity via the p38/JNK MAPK pathway[J].International Journal of Cardiovascular Disease,2020,01:48-51.
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黑芥子苷通过p38/JNK MAPK信号通路对阿霉素心脏毒性的保护作用(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2020年01期
页码:
48-51
栏目:
基础研究
出版日期:
2020-01-14

文章信息/Info

Title:
Sinigrin protects against doxorubicin-induced cardiotoxicity via the p38/JNK MAPK pathway
作者:
吕瑾 杨胜祥 华晓芳 刘长召
445000恩施土家族苗族自治州中心医院心血管内科
Author(s):
Lv Jin;  YANG Shengxiang;  HUA Xiaofang;  LIU Changzhao
Department of Cardiology, The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture, Hubei445000, China
关键词:
阿霉素;  心脏毒性;  黑芥子苷;  凋亡;  信号通路
Keywords:
Doxorubicin;  Cardiotoxicity;  Sinigrin;  Apoptosis;  Signaling pathway
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2020.01.012
文献标识码:
-
摘要:
目的:探讨黑芥子苷对阿霉素诱导的心脏毒性的作用及机制。方法:10周龄雄性C57/BL6小鼠随机分为对照组、黑芥子苷组、阿霉素组和黑芥子苷+阿霉素组。阿霉素组和黑芥子苷+阿霉素组给予单次腹腔注射阿霉素(15 mg/kg),对照组和黑芥子苷组给予单次腹腔注射生理盐水(15 mg/kg),黑芥子苷组和黑芥子苷+阿霉素组小鼠同时用黑芥子苷5 mg/kg灌胃,每2天1次,共3次。7 d后超声心动图检测心脏功能,Western blot检测心肌中凋亡相关蛋白和MAPK信号通路相关蛋白的表达水平。结果:黑芥子苷组与对照组小鼠超声心动图各指标、凋亡相关蛋白表达水平和MAPK相关蛋白表达水平的差异无统计学意义。与对照组相比,阿霉素组小鼠LVEDD、LVESD均明显增大,LVEF和LVFS均明显减小,Bax表达水平、p38和JNK的磷酸化水平明显升高,Bcl-2表达水平明显降低(P均<0.05)。与阿霉素组相比,黑芥子苷+阿霉素组小鼠LVEDD、LVESD均明显减小,LVEF和LVFS均明显增大,Bax表达水平、p38和JNK的磷酸化水平均明显降低,Bcl-2表达水平明显升高(P均<0.05)。结论:黑芥子苷通过p38/JNK MAPK信号通路减少心肌细胞凋亡,减轻阿毒素诱导的心脏毒性。
Abstract:
Objective:To investigate the effect and mechanism of sinigrin on doxorubicin-induced cardiotoxicity.Methods:Male C57/BL mice aged 10 weeks were randomly divided into control group, sinigrin group, doxorubicin group and sinigrin+doxorubicin group. A single intraperitoneal injection of doxorubicin(15 mg/kg)was given to mice in the doxorubicin group and the sinigrin+doxorubicin group. While in the control group and the mycosidin group, a single intraperitoneal injection of saline(15 mg/kg)was given. At the same time mice in the sinigrin group and the sinigrin+doxorubicin group were intragastrically administered with sinigrin 5 mg/kg once every 2 days, 3 times in total. After 7 days, cardiac function was evaluated by echocardiography, and the expression levels of apoptosis-related proteins and MAPK signaling pathway-related proteins in myocardium were detected by western blot.Results:There were no significant statistical differences in the echocardiographic indicators, expression of apoptosis-related proteins and MAPK-related proteins between the sinigrin group and the control group. Compared with the control group, LVEDD and LVESD were significantly larger, LVEF and LVFS were significantly smaller; Bax expression level, phosphorylation levels of P38 and JNK were significantly higher, and Bcl-2 expression level was significantly lower, in the doxorubicin group(P all<0.05). Compared with the doxorubicin group, LVEDD and LVESD were significantly smaller in the sirolimus+doxorubicin group, and LVEF and LVFS were significantly larger; Bax expression level, phosphorylation levels of P38 and JNK were significantly lower, while Bcl-2 expression levels were significantly higher(P all <0.05).Conclusions:Sinigrin reduces the cardiomyocyte apoptosis through P38/JNK MAPK signaling pathway and protects against doxorubicin-induced cardiotoxicity.

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备注/Memo

备注/Memo:
作者单位:445000恩施土家族苗族自治州中心医院心血管内科
通信作者:刘长召,E-mail:372465257@qq.com
更新日期/Last Update: 2020-01-15