|本期目录/Table of Contents|

[1]雍文兴 张卫强 张志明 刘永琦 颜春鲁 邵向阳 颉志英.不同缺血时间窗对大鼠在体心肌缺血再灌注损伤的影响[J].国际心血管病杂志,2019,03:146-149.
　YONG Wenxing,ZHANG Weiqiang,ZHANG Zhiming,et al.Effects of different ischemic time on ischemic-reperfusion injury in the rat heart in vivo[J].International Journal of Cardiovascular Disease,2019,03:146-149.
点击复制

不同缺血时间窗对大鼠在体心肌缺血再灌注损伤的影响(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2019年03期
页码:
146-149
栏目:
基础研究
出版日期:
2019-05-27

文章信息/Info

Title:
Effects of different ischemic time on ischemic-reperfusion injury in the rat heart in vivo
作者:
雍文兴 张卫强 张志明 刘永琦 颜春鲁 邵向阳 颉志英
730000 兰州,甘肃中医药大学附属医院急诊科(雍文兴,张志明); 730000 兰州,甘肃中医药大学(张卫强,刘永琦,颜春鲁,邵向阳,颉志英)
Author(s):
YONG Wenxing1;  ZHANG Weiqiang2;  ZHANG Zhiming1;  LIU Yongqi2;  YAN Chunlu2;  SHAO Xiangyang2;  XIE Zhiying2
1.Department of Emergency, Affiliated Hospital of Gansu University of Traditional Chinese Medicine, GanSu 730000; 2.Gansu University of Chinese Medicine, GanSu 730000, China
关键词:
大鼠;  缺血时间;  心肌缺血再灌注损伤
Keywords:
Rat;  Ischemic time;  Myocardial ischemic-reperfusion injury
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2019.03.005
文献标识码:
-
摘要:
目的:探讨不同缺血时间窗对大鼠在体心肌缺血再灌注损伤的影响。方法:取7～8周龄Wistar大鼠48只,雌雄各半,随机分为4组(假手术组、缺血7.5 min组、缺血15 min组、缺血30 min组),假手术组开胸后只穿线不结扎,其余各组开胸后分别结扎左冠状动脉前降支(LAD)7.5 min、15 min、30 min后行再灌注,术后检测大鼠心肌酶水平、心肌梗死面积、心功能情况,并行心肌病理检查。结果:与假手组比较,缺血7.5 min、15 min、30 min组血清心肌酶CK-MB含量均明显升高(P均<0.05),缺血15 min、30 min组心肌梗死面积均明显增大(P均<0.01); 超声心动图示缺血7.5 min组心功能与假手术组相比无变化(P>0.05),缺血15 min、30 min组心功能较假手术组均明显下降(P均<0.05); 心肌病理检查示各缺血组均有炎性细胞浸润,心肌结构损伤,缺血15 min、30 min组损伤更明显。结论:不同缺血再灌注时间对大鼠在体心肌损伤的影响不同。
Abstract:
Objective:To observe the effects of different time windows on myocardial ischemia-reperfusion injury in rats in vivo.Methods:Forty-eight Wistar rats aged 7～8 weeks with half male were randomly divided into four groups: sham-operated group, ischemic 7.5 min group, ischemic 15 min group and ischemic 30 min group. All the rats underwent thoracotomy. In ischemic groups, reperfusion was performed after ligating the left anterior descending coronary artery(LAD)for 7.5 min, 15 min and 30 min respectively. Myocardial enzyme levels, infarct size and ventricular function were measured after operation. Myocardial pathological examination was also performed.Results:Compared with sham-operated group, CK-MB level in ischemic groups increased significantly(all P<0.05), and infarct size was larger in ischemic 15 min group and ischemic 30 min group(all P<0.01). Echocardiography suggested that ventricular function decreased in ischemic 15 min and 30 min group contrast with sham-operated group(all P<0.05), while there was no significant change in ischemic 7.5 min group(P>0.05). Myocardial pathological examination showed that ischemic groups had inflammatory cell infiltration and myocardial structural damage, especially in the ischemic 15 min and 30 min group.Conclusions:Different ischemia-reperfusion time has different effects on myocardial injury in rats.

参考文献/References

[1] 唐丹丽, 崔海峰, 隋宇, 等. 痰瘀同治方对心肌缺血再灌注损伤大鼠核因子-κB通路及心肌自噬、凋亡的影响[J]. 中国中医药信息杂志, 2015, 22(12):38-42.
[2] 于佳慧, 李玉红, 段真珍, 等. 不同缺血再灌注时间对大鼠离体心脏缺血/再灌注损伤的影响[J]. 中国药学杂志, 2014, 49(18):1605-1609.
[3] 崔新明, 曹霞, 谷欣权, 等. 在体大鼠心肌缺血时间对再灌注损伤形成的影响[J]. 中国实验诊断学, 2010, 14(3):340-342.
[4] Storrow AB, Gibler WB. Chest pain centers: diagnosis of acute coronary syndromes[J]. Ann Emerg Med, 2000, 35(5):449-461.
[5] Kakimoto Y, Tsuruyama T, Miyao M, et al. The effectiveness and limitations of triphenyltetrazolium chloride to detect acute myocardial infarction at forensic autopsy[J]. Am J Forensic Med Pathol, 2013, 34(3):242-247.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81660730)
通信作者:张志明,Email:zhangzhimingys@163.com
更新日期/Last Update: 2019-05-27