|本期目录/Table of Contents|

[1]刘珊珊,王浩彦,周秀梅.血管紧张素Ⅱ在缺氧诱导的人肺成纤维细胞表型转化及胶原合成中的作用[J].国际心血管病杂志,2016,04:227-230.
　LIU Shanshan,WANG Haoyan,ZHOU Xiumei. . Department of Respiratory Medicine,et al.Effect of angiotensin Ⅱ on hypoxia-induced phenotype switch and collagen synthesis of human lung fibroblasts[J].International Journal of Cardiovascular Disease,2016,04:227-230.
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血管紧张素Ⅱ在缺氧诱导的人肺成纤维细胞表型转化及胶原合成中的作用(PDF)

《国际心血管病杂志》[ISSN:1006-6977/CN:61-1281/TN]

期数:
2016年04期
页码:
227-230
栏目:
出版日期:
2016-07-20

文章信息/Info

Title:
Effect of angiotensin Ⅱ on hypoxia-induced phenotype switch and collagen synthesis of human lung fibroblasts
作者:
刘珊珊; 王浩彦; 周秀梅
100071 北京丰台医院呼吸科(刘珊珊,周秀梅); 100050 首都医科大学附属北京友谊医院呼吸科(王浩彦)
Author(s):
LIU Shanshan1;  WANG Haoyan2;  ZHOU Xiumei1. 1. Department of Respiratory Medicine;  Beijing Fengtai Hospital;  Beijing 100071; 2. Department of Respiratory Medicine;  Beijing Friendship Hospital;  Capital Medical University;  Be
1. Department of Respiratory Medicine, Beijing Fengtai Hospital, Beijing 100071; 2. Department of Respiratory Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
关键词:
血管紧张素Ⅱ; 血管紧张素Ⅱ1型受体; 缺氧; 人肺成纤维细胞; 表型转化; Ⅰ型胶原蛋白
Keywords:
Angiotensin Ⅱ;  Angiotensin Ⅱ type 1 receptor;  Hypoxia;  Human lung fibroblast;  Phenotype switch;  Collagen typeⅠ
分类号:
-
DOI:
10.3969/j.issn.1673-6583.2016.04.011
文献标识码:
-
摘要:
目的:探讨血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)在缺氧诱导的人肺成纤维细胞(humanlungfibroblast,HLF)表型转化及胶原合成中的作用。方法:在缺氧条件下培养HLF-1细胞株,将细胞分为AngⅡ组、AngⅡ+替米沙坦(TST)组和对照组。采用免疫荧光法检测HLF-1细胞α-平滑肌肌动蛋白(α-smoothmuscleactin,α-SMA)的表达水平;采用Westernblot法检测HLF-1细胞Ⅰ型胶原(collagentypeⅠ,Col-Ⅰ)蛋白的表达水平。结果:AngⅡ组
Abstract:
Objective:To explore the effect of angiotensin Ⅱ(AngⅡ)on hypoxia-induced phenotype switch and collagen synthesis of human lung fibroblasts(HLF).Methods:The HLF-1 cell line was cultured in hypoxic condition,and randomly divided into three groups: control g

参考文献/References

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备注/Memo

备注/Memo:
通信作者:刘珊珊,Email:liushanshan721cn@126.com
更新日期/Last Update: 2016-07-20